A 42-year-old woman presents to the emergency department with symptoms of fatigue, nausea, and confusion. Laboratory tests reveal elevated ammonia levels, suggesting a defect in amino acid metabolism. Further testing indicates a deficiency in an enzyme that normally transfers an amino group from aspartate to α-ketoglutarate, forming glutamate and oxaloacetate. The enzyme follows a ping-pong Bi-Bi reaction mechanism. Which of the following best describes the enzymatic mechanism involved in this patient’s condition?
